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The partial specific volume v i ¯ , {\displaystyle {\bar {v_{i}}},} express the variation of the extensive volume of a mixture in respect to composition of the masses. It is the partial derivative of volume with respect to the mass of the component of interest.
where v i ¯ {\displaystyle {\bar {v_{i}}}} is the partial specific volume of a component i {\displaystyle i} defined as:
The PSV is usually measured in milliLiters per gram , proteins > 30 kDa can be assumed to have a partial specific volume of 0.708 mL/g. Experimental determination is possible by measuring the natural frequency of a U-shaped tube filled successively with air, buffer and protein solution.