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A Spot-tag is a 12-amino acid peptide tag recognized by a single-domain antibody. Due to the small size of a Spot-tag and the robust Spot-nanobody that specifically binds to Spot-tagged proteins, Spot-tag can be used for multiple capture and detection applications: Immunoprecipitation, affinity purification, immunofluorescence, and super-resolution microscopy. Recombinant proteins can be engineered to express the Spot-tag.
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