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In enzymology, a mandelamide amidase is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are -mandelamide and H2O, whereas its two products are -mandelate and NH3.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is mandelamide hydrolase. This enzyme is also called Pseudomonas mandelamide hydrolase.

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