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In enzymology, a pyridoxal phosphatase is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are pyridoxal 5'-phosphate and H2O, whereas its two products are pyridoxal and phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is pyridoxal-5'-phosphate phosphohydrolase. Other names in common use include vitamine B6 phosphatase, PLP phosphatase, vitamin B6-phosphate phosphatase, and PNP phosphatase. This enzyme participates in vitamin B6 metabolism.

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