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In enzymology, an aminoacyl-tRNA hydrolase is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are N-Substituted aminoacyl-tRNA and H2O, whereas its two products are N-substituted amino acid and tRNA.
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is aminoacyl-tRNA aminoacylhydrolase. Other names in common use include aminoacyl-transfer ribonucleate hydrolase, N-substituted aminoacyl transfer RNA hydrolase, and peptidyl-tRNA hydrolase.
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