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Di-tert-butoxyacetylene is the organic compound with the formula 3COC≡COC3. A pale yellow liquid, it is one of the more common dialkoxyacetylenes. Most other dialkoxyacetylenes are very labile. For example, dimethoxyacetylene polymerizes within seconds at 0 °C. Di-tert-butoxyacetylene can be prepared from 1,4-dioxane in a multistep procedure.

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