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Bicinchoninic acid /baɪsɪnkɔːnɪnɪk/ is a weak acid composed of two carboxylated quinoline rings.
Bicinchoninic is an organic compound with the formula 2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.
Bicinchoninic acid is most commonly employed in the bicinchoninic acid assay, which is used to determine the total level of protein in a solution. Bicinchoninic acid is used to analyze to monitor cuprous ion presence due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.
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