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The FOX reagent, or ferrous oxidation−xylenol orange, is used to measure levels of hydrogen peroxide in biological systems. The reagent is incubated with the sample and absorbance of the product form after a series of oxidation reactions is then measured at a wavelength of 560 nm. The reagent itself is an aqueous solution of ferrous ammonium sulfate, sorbitol, sulfuric acid and xylenol orange.
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