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5-enolpyruvylshikimate-3-phosphate synthase is an enzyme produced by plants and microorganisms. EPSPS catalyzes the chemical reaction:

Thus, the two substrates of this enzyme are phosphoenolpyruvate and 3-phosphoshikimate, whereas its two products are phosphate and 5-enolpyruvylshikimate-3-phosphate.

This enzyme is not present in animals. It presents an attractive biological target for herbicides, such as glyphosate. A glyphosate-resistant version of this gene has been used in genetically modified crops.

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