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Tetrasodium trisruthenium 3] is a sodium salt of coordination compound. In this form, it is the salt of a sulfonic acid. This compound is an extension of the phenanthroline series of coordination compounds. Ruthenium tris, referring to the anionic fragment, is used as a protein dye in biochemistry for differentiating and detecting different proteins in laboratory settings.
In recent years, 2-D electrophoresis has been widely accepted as a standard procedure to separate complex protein mixtures in proteome studies. Protein visualisation by Ruthenium tris has become a firmly established and widely used method in proteomic analysis and a crucial step in gene expression profiling.
For protein detection, it is advantageous to use fluorescent labels containing chromophores which have longer excitation wavelength and emission wavelength than the aromatic amino acids. The dyes used for this important step should combine attributes like good signal to background ratio , broad linear dynamic range, broad application range, photochemical stability and compatibility to protein identification techniques, e.g. mass spectrometry or Western blotting.
Originally, the ruthenium transition metal complex, ruthenium tris also termed as ruthenium tris was synthesized by Bannwarth as a precursor molecule for a dye that was used as a non-radioactive label for oligo nucleotides. Later, Rabilloud et al. used RuBPS as a fluorescent label for protein detection in polyacrylamide gels. The fact that RuBPS is not only easy to synthesize but also easy to handle, induced further developments in this field.