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The Colombia–Panama border is the 339-kilometer-long international boundary between Colombia and Panama. It also splits the Darién Gap, a break across the South American and North American continents. This large watershed, forest, and mountainous area is in the north-western portion of Colombia's Chocó Department and south-eastern portion of Panama's Darién Province.

There is also a gap in the Pan-American Highway that begins in Turbo, Colombia, and ends in Yaviza, Panama, and is 106 km long. Road-building through this area is expensive and the environmental cost is high, and no political consensus in favour of road construction has emerged.

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