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In enzymology, a hydroxybutyrate-dimer hydrolase is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are -3--3-hydroxybutanoyloxy]butanoate and H2O, whereas its product is -3-hydroxybutanoate.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is -3--3-hydroxybutanoyloxy]butanoate hydroxybutanoylhydrolase. This enzyme is also called D--3-hydroxybutyrate-dimer hydrolase. This enzyme participates in butanoate metabolism.

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