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Enoyl-CoA- isomerase isomerase, 3,2-trans-enoyl-CoA isomerase, ∆3,∆2-enoyl-CoA isomerase, or acetylene-allene isomerase, is an enzyme that catalyzes the conversion of cis- or trans-double bonds of coenzyme A bound fatty acids at gamma-carbon to trans double bonds at beta-carbon as below:

This enzyme has an important role in the metabolism of unsaturated fatty acids in beta oxidation.

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