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Isopenicillin N synthase is a non-heme iron protein belongig to the 2-oxoglutarate -dependent dioxygenases oxidoreductase family. This enzyme catalyzes the formation of isopenicillin N from δ--L-cysteinyl-D-valine.

This reaction is a key step in the biosynthesis of penicillin and cephalosporin antibiotics.

The active sites of most isopenicillin N synthases contain an iron ion.

This enzyme is also called isopenicillin N synthetase.

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