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Deoxyadenosine triphosphate is a nucleotide used in cells for DNA synthesis , as a substrate of DNA polymerase. It is classified as a purine nucleoside triphosphate, with its chemical structure consisting of a deoxyribose sugar molecule bound to an adenine and to three phosphate groups. It differs from the energy-transferring molecule adenosine triphosphate by a single hydroxyl group , resulting in a deoxyribose instead of a ribose. Two phosphate groups can be hydrolyzed to yield deoxyadenosine monophosphate, which can then be used to synthesize DNA.

Findings have also suggested that dATP can act as an energy-transferring molecule to maintain cell viability.

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