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In chemistry and biochemistry,the Henderson–Hasselbalch equation

relates the pH of a chemical solution of a weak acid to the numerical value of the acid dissociation constant, Ka, of acid and the ratio of the concentrations, {\displaystyle {\frac {}{}}} of the acid and its conjugate base in an equilibrium.

For example, the acid may be acetic acid

The Henderson–Hasselbalch equation can be used to estimate the pH of a buffer solution by approximating the actual concentration ratio as the ratio of the analytical concentrations of the acid and of a salt, MA.

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