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The pectinate line is a line which divides the upper two-thirds and lower third of the anal canal. Developmentally, this line represents the hindgut-proctodeum junction.

It is an important anatomical landmark in humans, and several distinctions can be made based upon the location of a structure relative to this line:

Microscopic cross section of the anorectal junction.

Anatomy of the anus and rectum

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