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In biological morphology and anatomy, a sulcus is a furrow or fissure. It may be a groove, natural division, deep furrow, elongated cleft, or tear in the surface of a limb or an organ, most notably on the surface of the brain, but also in the lungs, certain muscles , as well as in bones, and elsewhere. Many sulci are the product of a surface fold or junction, such as in the gums, where they fold around the neck of the tooth.

In invertebrate zoology, a sulcus is a fold, groove, or boundary, especially at the edges of sclerites or between segments.

In pollen a grain that is grooved by a sulcus is termed sulcate.

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