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Smart ligands are affinity ligands selected with pre-defined equilibrium , kinetic and thermodynamic parameters of biomolecular interaction.
Ligands with desired parameters can be selected from large combinatorial libraries of biopolymers using instrumental separation techniques with well-described kinetic behaviour, such as kinetic capillary electrophoresis , surface plasmon resonance , microscale thermophoresis , etc. Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed.
Smart ligands can find a set of unique applications in biomedical research, drug discovery and proteomic studies. For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide dynamic range of measured concentrations.