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The Warthin–Starry stain is a silver nitrate-based staining method used in histology. It was first introduced in 1920 by American pathologists Aldred Scott Warthin and Allen Chronister Starry , for the detection of spirochetes. It has been considered a standard stain for the detection of spirochetes, and is also used to stain Helicobacter pylori, Lawsonia intracellularis, Microsporidia, and particulates. It is also important for confirmation of Bartonella henselae, a causative organism in cat-scratch disease.

Warthin–Starry stains organisms dark brown to black, and the background light golden brown/golden yellow.

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