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Option 4 : 1, 2 and 3
The correct option is 1, 2, and 3.
Biofilm
- Biofilm is an association of micro-organisms in which microbial cells adhere to each other on living or non-living surfaces within a self-produced matrix of extracellular polymeric substances.
- Biofilm formation is a multi-step process starting with attachment to a surface then the formation of a micro-colony that leads to the formation of a three-dimensional structure and finally ending with maturation followed by detachment.
- Biofilms are ubiquitous and they form on virtually all surfaces immersed in a natural aqueous environment, e.g., water pipes, living tissue, tooth surface, implanted medical devices, dental implants, etc. Hence, statement 1 is correct.
- Diverse microorganisms are able to grow on food matrixes and along with food industry infrastructures. Thus biofilm can form on food and food processing surfaces. For example Escherichia coli, Salmonella enterica etc. Salmonella enterica contaminates a food pipeline biofilm and may induce massive outbreaks and even death in children and the elderly. Hence, statement 2 is correct.
- A biofilm is a structured consortium of bacteria embedded in a self-produced polymer matrix consisting of polysaccharides, proteins, and DNA. Bacterial biofilms show increased tolerance to antibiotics and disinfectant chemicals as well as resisting phagocytosis and other components of the body’s defense system. Thus they cause chronic infections. Hence, statement 3 is correct.
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