Structural Study MCQ

Test your knowledge with important Structural Study MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Structural Study MCQs at Bissoy

• Sometimes intact charged protein generated by ESI is introduced into the mass analyzer and is subjected to gas-phase fragmentation. What is this type of approach called?
  ক

  “Fragmentation” strategy of protein analysis

  খ

  “Left-to-right” strategy of protein analysis

  গ

  “Bottom-up” strategy of protein analysis

  ঘ

  “Top-down” strategy of protein analysis
• ESI-MS can successfully detect post-translational modifications and mutations.
  ক

  False

  খ

  True
• Which of the following is not an application of ESI-MS?
  ক

  Protein identification and characterization

  খ

  In the clinical laboratory

  গ

  Studying non-covalent interactions

  ঘ

  Studying covalent interactions
• In which of the following techniques the gaseous ions are subjected to two or more sequential stages of mass analysis according to the m/z ratio?
  ক

  NMR spectroscopy

  খ

  X-ray crystallography

  গ

  Mass spectrometry

  ঘ

  Tandem mass spectrometry
• In ESI-MS vacuum should be maintained in which of the following components?
  ক

  Detector only

  খ

  Mass analyzer only

  গ

  Ion source and mass analyzer

  ঘ

  Mass analyzer and detector
• Which of the following is not a component of ESI-MS mass spectrometry?
  ক

  Electrospray ionizer

  খ

  Detector

  গ

  Mass analyzer

  ঘ

  MALDI
• Who is the inventor of ESI-MS?
  ক

  Benjamin Franklin

  খ

  Franz H. Kemp

  গ

  Michael Barber

  ঘ

  John B. Fenn
• Generally in ESI, very little residual energy is retained by the analyte, and no fragmentation occurs upon ionization.
  ক

  False

  খ

  True
• Which of the following ionization technique was able to overcome the propensity of the analyte fragmentation?
  ক

  Electron ionization

  খ

  FAB

  গ

  Chemical ionization

  ঘ

  ESI
• What is ESI is also known?
  ক

  Standard Pressure Ionization (SPI)

  খ

  High-Pressure Ionization (HPI)

  গ

  Low-Pressure Ionization (LPI)

  ঘ

  Atmospheric Pressure Ionization (API)
• The ions created in ESI are accelerated towards mass analyzer depending upon which criteria of ions?
  ক

  Mass of the ion

  খ

  Charge on the ion

  গ

  Weight of the ion

  ঘ

  Mass and charge of the ion
• What is the essential principle in ESI?
  ক

  Solubilization

  খ

  Nucleation

  গ

  Sublimation

  ঘ

  Nebulisation
• ESI technique does not allow the analysis of large (underivatized) biomolecules, such as proteins and DNA.
  ক

  True

  খ

  False

  গ

  3. ESI technique does not allow the analysis of large (underivatize

  ঘ

  biomolecules, such as proteins and DNA. a) True b) False
• ESI is not a soft ionization technique.
  ক

  True

  খ

  False
• Which of the following ionization techniques involves the production of ions by spraying a solution of the analyte into an electric field?
  ক

  CI

  খ

  MALDI

  গ

  FAB

  ঘ

  ESI
• Which of the following lasers can be used for laser radiation in MALDI?
  ক

  Infrared lasers

  খ

  Greenlight lasers

  গ

  Red light lasers

  ঘ

  Ultraviolet lasers
• Which of the following provides higher resolution in MALDI-TOF?
  ক

  Blocker

  খ

  Channel

  গ

  Transmitter

  ঘ

  Reflector
• Which of the following cannot be used as a MALDI matrix compound?
  ক

  α-Cyano-4-hydroxycinnamic acid

  খ

  Sinapinic acid

  গ

  3-Hydroxypicolinic acid

  ঘ

  2,3-Dihydroxybenzoic acid
• In TOF, ions of lower mass are accelerated to lower velocities and reach the detector last.
  ক

  True

  খ

  False
• Which of the following type of mass analyzer uses a magnetic field and cyclotron motion to trap ions?
  ক

  Quadrupole

  খ

  Orbitrap

  গ

  TOF

  ঘ

  Fourier transform ion cyclotron resonance
• What is the relation of time of flight with the molecular mass of the ion in the TOF mass analyzer?
  ক

  Time of flight is inversely proportional to the square root of the molecular mass of the ion

  খ

  Time of flight is inversely proportional to the molecular mass of the ion

  গ

  Time of flight is directly proportional to the molecular mass of the ion

  ঘ

  Time of flight is directly proportional to the square root of the molecular mass of the ion
• In the TOF analyzer, all the ions are accelerated at the same velocity.
  ক

  True

  খ

  False
• Which of the following ionization method uses aromatic compounds and organic solvents to achieve ionization?
  ক

  ESI

  খ

  Chemical ionization

  গ

  FAB

  ঘ

  MALDI
• Sublimation occurs in which of the following ionization methods?
  ক

  ESI

  খ

  FAB

  গ

  Chemical Ionization

  ঘ

  MALDI
• Which of the following ionization technique uses a short laser pulse to achieve ionization?
  ক

  ESI

  খ

  Chemical Ionization

  গ

  FAB

  ঘ

  MALDI
• Which mass analyzer is most frequently used with MALDI?
  ক

  Magnetic sector

  খ

  Quadrupole

  গ

  Ion trap

  ঘ

  TOF
• What is the monoisotopic mass of proline? Given data: Mass of the most abundant isotope of Carbon (C°)=12.0000, Mass of most abundant isotope of Hydrogen (H°)=1.0078, Mass of most abundant isotope of Oxygen (O°)=15.9949, Mass of most abundant isotope of Nitrogen (N°)=14.0031.
  ক

  91.527

  খ

  43.519

  গ

  87.063

  ঘ

  97.052
• Which of the following type of mass analyzer makes use of a combination of electric and magnetic fields and captures ions in a region of vacuum system?
  ক

  Quadrupole mass analyzer

  খ

  Ion cyclotron resonance

  গ

  TOF

  ঘ

  Ion trap mass analyzer
• Which of the following can be used as a detector as well as an analyzer in Mass spectrometry?
  ক

  Conversion diode

  খ

  Array detector

  গ

  Electron multiplier

  ঘ

  Ion cyclotron Fourier transform
• Which of the following is not a soft ionization technique?
  ক

  ESI

  খ

  FAB

  গ

  MALDI

  ঘ

  CI