Plant Biotechnology MCQ

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• Determine the correctness or otherwise of the following Assertion (A) and the Reason (R).<br>Assertion: Somatic embryogenesis in plants is a twostep process comprising of embryo initiation followed by embryo production.<br>Reason: Embryo initiation is independent of the presence of 2, 4-dichlorophenoxyacetic acid, whereas embryo production requires a high concentration of 2, 4-dichlorophenoxyacetic acid.
  ক

  Both (A) and (R) are true and (R) is the correct reason for (A)

  খ

  Both (A) and (R) are true and (R) is not the correct reason for (A)

  গ

  (A) is true but (R) is false

  ঘ

  (A) is false but (R) is true
• Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of
  ক

  Sodium alginate and dropping it in a solution of calcium nitrate

  খ

  Calcium alginate and dropping it in a solution of sodium nitrate

  গ

  Calcium alginate and dropping it in a solution of ammonium nitrate

  ঘ

  Mannitol and dropping it in a solution of sodium nitrate
• Match the secondary metabolites (Group I) with the corresponding plant species (Group II).<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. Morphine</td> <td>1. Datura stramonium</td> </tr> <tr> <td>Q. Pyrethrins</td> <td>2. Catharanthus roseus</td> </tr> <tr> <td>R. Scopolamine</td> <td>3. Papaver somniferum</td> </tr> <tr> <td>S. Vincristine</td> <td>4. Tagetes erecta</td> </tr></table> P-4, Q-3, R-1, S-2

  খ

  P-3, Q-4, R-1, S-2

  গ

  P-2, Q-3, R-4, S-1

  ঘ

  P-4, Q-1, R-2, S-3
• Agrobacterium based transformation of protoplasts obtained from dicots is based on the fact that
  ক

  These exhibit strong chromosomal structures

  খ

  These have two cotyledons

  গ

  These exhibits strong wound response

  ঘ

  These have long tap root system
• Vincristine and vinblastine, two commercially important secondary metabolites from Catharanthus roseus, are examples of
  ক

  Alkaloids

  খ

  Flavonoids

  গ

  Terpenoids

  ঘ

  Steroids
• Expression of antisense RNA of ACC synthase in transgenic tomato plants inhibited the synthesis of ethylene resulting in
  ক

  Change in color from green to red

  খ

  Change in aroma

  গ

  Charge in color from red to green

  ঘ

  None of the above
• Virus resistant transgenic plants can be developed by the expression of
  ক

  Cowpea trypsin inhibitor

  খ

  Crystalline toxin protein

  গ

  Defective movement protein

  ঘ

  Snowdrop lectin
• To produce plants that are homozygous for all traits, the best choice is
  ক

  Protoplast culture

  খ

  Cell suspension culture

  গ

  Anther and pollen culture

  ঘ

  Apical meristem culture
• Plant secondary metabolites production in suspension culture is mainly targeted for
  ক

  Obtaining metabolites in aseptic condition

  খ

  Enhanced in vitro production of desired metabolite

  গ

  Enhanced production of all metabolites

  ঘ

  Obtaining new metabolites
• Match the herbicides in Group I with the target enzymes in Group II.<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. Glyphosate</td> <td>1. Nitrilase</td> </tr> <tr> <td>Q. Bromoxynil</td> <td>2. Acetolactatesynthetase</td> </tr> <tr> <td>R. Sulphonylureas</td> <td>3. Dehalogenase</td> </tr> <tr> <td>S. Dalapon</td> <td>4. 5-Enolpyruvyl shikimate<br>     3-phosphate synthase</td> </tr></table> P-4, Q-1, R-2, S-3

  খ

  P-2, Q-1, R-4, S-3

  গ

  P-4, Q-3, R-2, S-1

  ঘ

  P-3, Q-2, R-4, S-1
• Cells of meristemoid are best described as
  ক

  Differentiated and non-dividing

  খ

  Dedifferentiated and dividing

  গ

  Differentiated and dividing

  ঘ

  Dedifferentiated and non-dividing
• Somatic embryogenesis is a procedure in plant tissue culture methodology described best as
  ক

  Formation of both shoot and root meristem

  খ

  Formation of stable embryos

  গ

  Formation of axillary buds

  ঘ

  None of the above
• Some of the cytokinins used in plant tissue culture media are given below:<br>P. BAP<br>Q. Zeatin<br>R. Kinetin<br>S. 2iP<br>Which of these are synthetic analogs?
  ক

  P and Q only

  খ

  Q and S only

  গ

  R and S only

  ঘ

  P and R only
• Stable transformation of plants is reliably obtained by
  ক

  Agrobacterium plasmid integration

  খ

  Electroporation

  গ

  Microinjection

  ঘ

  Silicon carbide whisker
• The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. This is because of
  ক

  The coat of the intact seeds blocks the pungent volatiles from being released

  খ

  The pungent chemicals are stored as inactive conjugates and compartmentalized from the enzymes that convert them into active chemicals

  গ

  The pungent chemicals are formed only after the reaction with atmospheric oxygen

  ঘ

  The pungent chemicals are formed only after the reaction with atmospheric carbon dioxide
• Resistance to herbicide chlorsulfuron in plants is due to change in
  ক

  Glutamine synthetase

  খ

  Acetolactate synthase

  গ

  Theronine deaminase

  ঘ

  DNA polymerase
• Hormone pairs required for a callus to differentiate are
  ক

  Auxin and cytokinin

  খ

  Auxin and gibberellin

  গ

  Ethylene and gibberellin

  ঘ

  Cytokinin and gibberellin
• Match the following plant sources with their secondary metabolites and medical uses.<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Source plant</strong></td> <td><strong>Secondary metabolites</strong></td> <td><strong>Medical use</strong></td> </tr> <tr> <td>P. Belladonna</td> <td>1. Menthol</td> <td>a. Cancer treatment</td> </tr> <tr> <td>Q. Foxglove</td> <td>2. Atropine</td> <td>b. Heart disease</td> </tr> <tr> <td>R. Pacific yew</td> <td>3. Digitalin</td> <td>c. Eye examination</td> </tr> <tr> <td>S. Eucalyptus</td> <td>4. Taxol</td> <td>d. Cough</td> </tr></table> P-2-c, Q-3-b, R--4-a, S-1-d

  খ

  P-3-c, Q-2-a, R-1-d, S-4-b

  গ

  P-2-c, Q-4-b, R-1-a, S-3-d

  ঘ

  P-1-b, Q-4-c, R-2-d, S-3-a
• The plant hormone indole-3-acetic acid is derived from
  ক

  Histidine

  খ

  Tyrosine

  গ

  Tryptophan

  ঘ

  Proline
• Which of the following combinations of plant hormones and their associated function are correct?<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Hormone</strong></td> <td><strong>Function</strong></td> </tr> <tr> <td>P. Abscisic acid</td> <td>1. Breaks seed dormancy</td> </tr> <tr> <td>Q. Auxin</td> <td>2. Induces cell division</td> </tr> <tr> <td>R. Ethylene</td> <td>3. Stimulates ripening of fruits</td> </tr> <tr> <td>S. Gibberellin</td> <td>4. Promotes seed dormancy</td> </tr></table> P and R only

  খ

  P and S only

  গ

  Q and R only

  ঘ

  Q and S only
• Which one of the following is a second generation genetically engineered crop?
  ক

  Bt brinjal

  খ

  Roundup soyabean

  গ

  Golden rice

  ঘ

  Bt rice
• Multiplication of genetically identical copies of a cultivar by asexual reproduction is known as
  ক

  Aclonal propagation

  খ

  Vegetative propagation

  গ

  Polyclonal propagation

  ঘ

  Clonal propagation
• What would he the effect of addition of 2, 4-D on the production of berberine by cell culture of Thalictrum minus?
  ক

  To stimulate growth and thereby urease secondary metabolite production

  খ

  Stimulate dedifferentiation and thereby decrease secondary metabolite production

  গ

  Stimulate proliferation and reduce secondary metabolite production

  ঘ

  None of the above
• Which of the following statements is most appropriate for recombinant antibody production in transgenic plants?
  ক

  A very high level expression is always obtained

  খ

  Light promote more antibody production

  গ

  Such antibodies are free from other antigen of animal origin

  ঘ

  Functional antibody cannot be produced in plants
• The transplastomic plants bear no risk for gene transfer through pollens as
  ক

  The pollens degenerate before fertilization

  খ

  The transformed mitochondrial DNA is lost during pollen maturation

  গ

  The transformed chloroplast DNA is lost during pollen maturation

  ঘ

  The transformed genomic DNA are inherited maternally
• Match the promoters Listed in Group I with the tissues listed in Group II<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Group I</strong></td> <td><strong>Group II</strong></td> </tr> <tr> <td>P. α-Amylase</td> <td>1. Endosperm</td> </tr> <tr> <td>Q. Glutenin</td> <td>2. Tuber</td> </tr> <tr> <td>R. Phaseollin</td> <td>3. Aleurone</td> </tr> <tr> <td>S. Patatin</td> <td>4. Cotyledon</td> </tr></table> P-3, Q-1, R-4, S-2

  খ

  P-3, Q-4, R-1, S-2

  গ

  P-4, Q-2, R-1, S-3

  ঘ

  P-1, Q-3, R-2, S-4
• Which one of the following is the growth factor used for growth of tissues and organs in plant tissue culture?
  ক

  Cysteine

  খ

  Cytokinin

  গ

  Cytidylate

  ঘ

  Cyclic AMP
• Identify the natural plant growth regulators from the following list.<br>P. Zeatin<br>Q. Benzylaminopurine (BAP)<br>R. Indole-3-acetic acid (IAA)<br>S. 2, 4-Dichlorophenoxyacetic acid
  ক

  P, Q

  খ

  Q, S

  গ

  P, R

  ঘ

  R, S
• Callus formation from mature tissue explant occurs through
  ক

  Dedifferentiation

  খ

  Redifferentiation

  গ

  Both A and B of the above

  ঘ

  Neither A nor B
• When present in tissue culture medium, gibberellin
  ক

  Helps to break dormancy of buds and bulbs

  খ

  Promotes dormancy development in buds and bulbs

  গ

  Is regarded as plant growth inhibitor

  ঘ

  Prevents normal recognition of auxin molecule