ESI-MS MCQ

Test your knowledge with important ESI-MS MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more ESI-MS MCQs at Bissoy

• Sometimes intact charged protein generated by ESI is introduced into the mass analyzer and is subjected to gas-phase fragmentation. What is this type of approach called?
  ক

  “Fragmentation” strategy of protein analysis

  খ

  “Left-to-right” strategy of protein analysis

  গ

  “Bottom-up” strategy of protein analysis

  ঘ

  “Top-down” strategy of protein analysis
• ESI-MS can successfully detect post-translational modifications and mutations.
  ক

  False

  খ

  True
• Which of the following is not an application of ESI-MS?
  ক

  Protein identification and characterization

  খ

  In the clinical laboratory

  গ

  Studying non-covalent interactions

  ঘ

  Studying covalent interactions
• In which of the following techniques the gaseous ions are subjected to two or more sequential stages of mass analysis according to the m/z ratio?
  ক

  NMR spectroscopy

  খ

  X-ray crystallography

  গ

  Mass spectrometry

  ঘ

  Tandem mass spectrometry
• In ESI-MS vacuum should be maintained in which of the following components?
  ক

  Detector only

  খ

  Mass analyzer only

  গ

  Ion source and mass analyzer

  ঘ

  Mass analyzer and detector
• Which of the following is not a component of ESI-MS mass spectrometry?
  ক

  Electrospray ionizer

  খ

  Detector

  গ

  Mass analyzer

  ঘ

  MALDI
• Who is the inventor of ESI-MS?
  ক

  Benjamin Franklin

  খ

  Franz H. Kemp

  গ

  Michael Barber

  ঘ

  John B. Fenn
• Generally in ESI, very little residual energy is retained by the analyte, and no fragmentation occurs upon ionization.
  ক

  False

  খ

  True
• Which of the following ionization technique was able to overcome the propensity of the analyte fragmentation?
  ক

  Electron ionization

  খ

  FAB

  গ

  Chemical ionization

  ঘ

  ESI
• What is ESI is also known?
  ক

  Standard Pressure Ionization (SPI)

  খ

  High-Pressure Ionization (HPI)

  গ

  Low-Pressure Ionization (LPI)

  ঘ

  Atmospheric Pressure Ionization (API)
• The ions created in ESI are accelerated towards mass analyzer depending upon which criteria of ions?
  ক

  Mass of the ion

  খ

  Charge on the ion

  গ

  Weight of the ion

  ঘ

  Mass and charge of the ion
• What is the essential principle in ESI?
  ক

  Solubilization

  খ

  Nucleation

  গ

  Sublimation

  ঘ

  Nebulisation
• ESI technique does not allow the analysis of large (underivatized) biomolecules, such as proteins and DNA.
  ক

  True

  খ

  False

  গ

  3. ESI technique does not allow the analysis of large (underivatize

  ঘ

  biomolecules, such as proteins and DNA. a) True b) False
• ESI is not a soft ionization technique.
  ক

  True

  খ

  False
• Which of the following ionization techniques involves the production of ions by spraying a solution of the analyte into an electric field?
  ক

  CI

  খ

  MALDI

  গ

  FAB

  ঘ

  ESI