Enzyme Engineering In Biotechnology MCQ

Test your knowledge with important Enzyme Engineering In Biotechnology MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Enzyme Engineering In Biotechnology MCQs at Bissoy

• Increase or decrease in . . . . . . . . leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.
  ক

  Temperature

  খ

  Ionic strength

  গ

  pH

  ঘ

  Detergent use
• . . . . . . . . is a phenomenon of assuming frequencies more than 20 kHz which is above the human audibility.
  ক

  Homogenization

  খ

  Ultrasonics

  গ

  Alkali treatment

  ঘ

  Freezing
• Enzyme which can be extracted from mammary gland is . . . . . . . .
  ক

  Pectinase

  খ

  Glucornidase

  গ

  Lipase

  ঘ

  Acetyl CoA carboxylase
• Enzyme catalysing rearrangement of atomic grouping without altering molecular weight or number of atom is:
  ক

  Ligase

  খ

  Isomerase

  গ

  Oxidoreductase

  ঘ

  Hydrolase
• Homogenization utilizing . . . . . . . . is one where in manual pressure is created for cell lysis.
  ক

  Mortar and pestle

  খ

  Ultrasonicator

  গ

  Freezer

  ঘ

  French press
• This statement about enzymes is true:
  ক

  Enzymes accelerate reactions by lowering the activation energy

  খ

  Enzymes are proteins whose three-dimensional form is key to their function

  গ

  Enzymes do not alter the overall change in free energy for a reaction

  ঘ

  All of these
• The phenomenon wherein the osmotic pressure created due to presence of solutes inside and outside the cells will be equal is referred to as . . . . . . . .
  ক

  Isotonic condition

  খ

  Hypertonic condition

  গ

  Hypotonic condition

  ঘ

  Osmotic shock
• What is the rate of denaturation k_d, if the half-life of an enzyme catalyzed reaction is 15.6 mins?
  ক

  4.4*10^-2/sec

  খ

  0.4*10²/min

  গ

  4.4*10^-2/min

  ঘ

  0.4*10²/sec
• In this below equation, Enzyme Y belongs to which class of enzymes?<br>\[A - X + {H_2}O\xrightarrow[{Enzyme\,Y}
  ক

  {}X - OH + AH\

  খ

  <p><span>A.</span> Peroxidase

  গ

  </span> Hydrolases
• For every, 1000 fold increase in pressure, the K_m is . . . . . . . .
  ক

  Reduced to half

  খ

  Doubled

  গ

  Reduced to zero

  ঘ

  Increased by 10 folds
• Which type of restriction enzymes are commonly used in rDNA technology
  ক

  Type I

  খ

  Type II

  গ

  Type III

  ঘ

  Type IV
• An uncatalyzed reaction involved:
  ক

  High activation energy

  খ

  Low activation energy

  গ

  Balanced activation energy

  ঘ

  All of these
• Histidase: Lyases :: Chymosin: . . . . . . . .
  ক

  Ligases

  খ

  Isomerases

  গ

  Hydrolases

  ঘ

  Transferases
• Enzymes which are secreted outside the body cells are referred to as . . . . . . . . enzymes.
  ক

  Extracellular

  খ

  Intracellular

  গ

  Cytoplasmic

  ঘ

  Mitochondrial
• Substances which reduce the rate of enzyme catalyzed reactions are known as . . . . . . . .
  ক

  Substrates

  খ

  Enzymes

  গ

  Products

  ঘ

  Inhibitors
• Optimum pH value of pepsin is:
  ক

  1.4

  খ

  2

  গ

  3.5

  ঘ

  5
• In . . . . . . . . treatment, produced basic radicals mediate a nucleophilic attack on compounds of cell wall on membrane leading to destabilization and fragmentation of membrane followed by release of enzymes.
  ক

  EDTA

  খ

  Osmotic shock

  গ

  Alkali

  ঘ

  Detergent
• Klenow enzyme is the product of enzymatic breakdown of
  ক

  DNA polymerase I

  খ

  DNA polymerase II

  গ

  DNA polymerase III

  ঘ

  RNA polymerase
• Homogenization performed by passing the cells at high pressure through narrow orifice which can also cause cell rupture is referred to as . . . . . . . .
  ক

  Ultrasonicator

  খ

  Freezer

  গ

  Mortar and pestle

  ঘ

  French press
• The enzyme which specifically catalyzes β (1 → 4) glycosidic linkages of NAM (N-acetyl muramic acid) and NAG (N-acetyl glucosamine) present in bacterial peptidoglycan is . . . . . . . .
  ক

  Protease

  খ

  Lipase

  গ

  Lysozyme

  ঘ

  Cellulase
• Loss of activity which may be restored by the removal of inhibitor is referred to as . . . . . . . .
  ক

  Reversible inhibition

  খ

  Irreversible inhibition

  গ

  Competitive inhibition

  ঘ

  Mixed inhibition
• If K_a for acetic acid is 1.78*10^-5, the pK_a of acetic acid is . . . . . . . .
  ক

  0.475

  খ

  4.75

  গ

  2.5

  ঘ

  0.25
• . . . . . . . . is an enzyme, which is highly produced by egg white and lachrymal glands.
  ক

  Amylases

  খ

  Lysozyme

  গ

  Invertase

  ঘ

  Protease
• Enzyme complex involved in alcoholic fermentation is:
  ক

  Zymase

  খ

  Invertase

  গ

  Lipase

  ঘ

  Amylase
• The enzyme which hydrolyses starch to maltose is:
  ক

  Protease

  খ

  Amylase

  গ

  Lactase

  ঘ

  Maltase
• Which of these is correct for mixed inhibition?
  ক

  Lowers to 0.5 V_max and 0.5 K_m

  খ

  V_max is unchanged and K_m increases 2K_m

  গ

  Lowers to 0.5 V_max and K_m remains unchanged

  ঘ

  Lowers to 0.67 V_max and K_m increases to 2K_m
• What happens to V_max and K_m in case of uncompetitive inhibition when I = K_i'?
  ক

  Lowers to 0.5 V_max and 0.5 K_m

  খ

  V_max is unchanged and K_m increases 2K_m

  গ

  Lowers to 0.5 V_max and K_m remains unchanged

  ঘ

  Lowers to 0.67 V_max and K_m increases to 2K_m
• The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as . . . . . . . .
  ক

  Ultrasonics

  খ

  Freezing

  গ

  Homogenization

  ঘ

  Osmotic shock
• The pH at which the net charge on the enzyme molecule is zero is called . . . . . . . .
  ক

  pK_a

  খ

  Half-life

  গ

  Isoelectric point

  ঘ

  K_m
• Which of the following is an RNA dependent DNA synthetase
  ক

  DNA polymerase I

  খ

  DNA polymerase II

  গ

  Reverse transcriptase

  ঘ

  All of these