DNA Replication MCQ

Test your knowledge with important DNA Replication MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30+ more DNA Replication MCQs on Bissoy. Bissoy App

• Name the protein, which is responsible for the formation of RNA primer?
  ক

  Topoisomerase

  খ

  Gyrase

  গ

  Helicase

  ঘ

  Primase
• Which of the following would relax negatively supercoiled DNA?
  ক

  DNA gyrase

  খ

  Type I topoisomerase

  গ

  Collagen

  ঘ

  Elastin
• Name the protein, which is used for termination of replication?
  ক

  DnaC

  খ

  SSB

  গ

  Tus protein

  ঘ

  DNA polymerase
• Which of the following protein is required for connecting Okazaki fragments?
  ক

  Scaffold protein

  খ

  Helicase

  গ

  Primase

  ঘ

  DNA gyrase
• Which of the following protein does not involve in the initiation of replication?
  ক

  DnaA

  খ

  SSB (Single strand binding protein)

  গ

  DnaB

  ঘ

  DnaF
• How many numbers of replicon is found in E.coli?
  ক

  Five replicon

  খ

  Two replicon

  গ

  Single replicon

  ঘ

  Multiple replicon
• What is the origin of replication?
  ক

  Particular site at which DNA replication starts

  খ

  Site which prevents initiation

  গ

  Random location on the DNA

  ঘ

  Site at which replication terminated
• What is a mode of replication in E.coli?
  ক

  Intermediate

  খ

  Dispersive

  গ

  Conservative

  ঘ

  Semiconservative
• Base sequence of each parental strand considered to synthesis new complementary strand.
  ক

  True

  খ

  False
• Double-helix structure of DNA is discovered by___________
  ক

  Gobind Khurana

  খ

  Nirenberg

  গ

  Watson and Crick

  ঘ

  Darwin
• Which of the following enzyme is responsible for initiating DNA replication?
  ক

  polymerase

  খ

  ligase

  গ

  primase

  ঘ

  gyrase
• Which enzyme joins the Okazaki fragments into a continuous strand?
  ক

  gyrase

  খ

  topoisomerase

  গ

  ligase

  ঘ

  helicase
• Who made the discovery that one DNA strand is synthesized discontinuously in fragments?
  ক

  Francis Crick

  খ

  Reiji Okazaki

  গ

  James Watson

  ঘ

  Louis Pasteur
• The lagging strand is synthesized discontinuously.
  ক

  True

  খ

  False
• How many copies (approximately) of DNA polymerase III are present in a typical bacterial cell?
  ক

  10

  খ

  50

  গ

  100

  ঘ

  200
• When was DNA polymerase III discovered as the major enzyme behind DNA replication in bacteria?
  ক

  1949

  খ

  1959

  গ

  1969

  ঘ

  1989
• Which enzyme removes the positive supercoils generated when the replication fork?
  ক

  helicase

  খ

  endonuclease

  গ

  exonuclease

  ঘ

  gyrase
• Movement of the replication fork generates ________________ in the unreplicated portion of DNA.
  ক

  positive supercoils

  খ

  negative supercoils

  গ

  untranslated regions

  ঘ

  amino acid substitutions
• The bacterial oriC is the __________________
  ক

  specific sequence

  খ

  transcription factor

  গ

  enzyme

  ঘ

  polypeptide
• Temperature-sensitive mutants can not replicate their chromosomes at non-permissive temperatures.
  ক

  True

  খ

  False
• Which isotopes were used by the researchers who first confirmed the semiconservative nature of DNA replication?
  ক

  Nitrogen

  খ

  Carbon

  গ

  Oxygen

  ঘ

  Sulfur
• How many types of replication models were considered before the semiconservative nature of DNA replication was confirmed?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• Which enzyme used to join bits of DNA?
  ক

  DNA polymerase

  খ

  DNA ligase

  গ

  Endonuclease

  ঘ

  Primase
• Which of the following enzymes is the principal replication enzyme in E. coli?
  ক

  DNA polymerase I

  খ

  DNA polymerase II

  গ

  DNA polymerase III

  ঘ

  None of the mentioned
• DNA unwinding is done by ____________
  ক

  Ligase

  খ

  Helicase

  গ

  Topoisomerase

  ঘ

  Hexonuclease
• Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?
  ক

  5’ to 3’ exonuclease activity

  খ

  3’ to 5’ exonuclease activity

  গ

  3’ to 5’ endonuclease activity

  ঘ

  5’ to 3’ endonuclease activity
• What is the reaction in DNA replication catalyzed by DNA ligase?
  ক

  Addition of new nucleotides to the leading strand

  খ

  Addition of new nucleotide to the lagging strand

  গ

  Formation of a phosphodiester bond between the 3’-OH of one Okazaki fragment and the 5’-phosphate of the next on the lagging strand

  ঘ

  Base pairing of the template and the newly formed DNA strand
• Which of the following is true about DNA polymerase?
  ক

  It can synthesize DNA in the 5’ to 3’ direction

  খ

  It can synthesize DNA in the 3’ to 5’ direction

  গ

  It can synthesize mRNA in the 3’ to 5’ direction

  ঘ

  It can synthesize mRNA in the 5’ to 3’ direction
• Eukaryotes differ from prokaryote in mechanism of DNA replication due to ____________
  ক

  Use of DNA primer rather than RNA primer

  খ

  Different enzyme for synthesis of lagging and leading strand

  গ

  Discontinuous rather than semi-discontinuous replication

  ঘ

  Unidirectional rather than semi-discontinuous replication
• Semi-conservative DNA replication was first demonstrated in ____________
  ক

  Drosophila melanogaster

  খ

  Escherichia coli

  গ

  Streptococcus pneumonae

  ঘ

  Drosophila melanogaster