The length of linker DNA is

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

A

1, 2, 3 and 4 are true

B

Only 2 and 4 are true

C

Oniy 2, 3 and 4 are true

D

Only 1 and 2 are true

Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because

A

The sequence context at the RB defines the direction of transfer

B

The sequence context at the LB defines the direction of transfer

C

The nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand

D

The endonuclease activity of VirD2 protein allows nicking at RB

Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:

A

1, 2 and 3

B

3 and 4

C

1, 3 and 4

D

Only 4

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

A

3, 1, 2, 4, 6, 5, 7, 8

B

1, 4, 3, 2, 5, 7, 6, 8

C

1, 2, 6, 7, 5, 8, 3, 4

D

8, 6, 7, 5, 1 , 4, 2, 3

Select the correct differences between char and varchar data types? i. varchar is non unicode and char is unicode character data type ii. char is ‘n’ bytes whereas varchar is actual length in bytes of data entered in terms of storage size iii. varchar is variable in length and char is the fixed length string iv. For varchar, if a string is less than the maximum length then it is stored in verbatim without any extra characters while for char if a string is less than the set length it is padded with extra characters to equalize its length to given length

A

i, iii, iv

B

ii, iii, iv

C

i, ii, iv

D

iii, iv

Which of the following histones bind to linker DNA?

A

H1

B

H2A

C

H2B

D

H3

Consider the following statements regarding the DNA-mediated gene transfer and choose the correct answer:
1. DNA fragments carrying the gene to be transferred are mixed with a carrier DNA
2. They are then precipitated out with a calcium phosphate buffer
3. The precipitate is used to transform target cells
4. The Ca-precipitated DNA enters the cell by exocytosis

A

Only 1, 2 and 4 are true

B

Only 3 and 4 are true

C

Only 2, 3 and 4 life true

D

Only 1, 2 and 3 are true

Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease

A

1, 2, 3 and 4 are true

B

Only 2, 3 and 4 are true

C

Only 1, 2 and 3 are true

D

Only 1, 3 and 4 are true

Consider the following statements regarding satellite DNA and choose the correct answer:
1. Satellite or Repetitive DNA consists of short identical genes which are repeated in tandem several hundred or thousand times
2. Such DNA is found in the region of the chromosome adjacent to the centromere
3. It is easy to separate repetitive DNA by ultracentrifugation

A

Only 1 and 2 are true

B

Only 2 and 3 are true

C

Only 1 and 3 are true

D

1, 2 and 3 are true

Which of following words is not used as a linker?

A

so

B

namely

C

budget

D

too

The length of linker DNA is

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