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A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain of E. coli having the genotype lacI+ crp+. Even in the absence of inducer IPTG, low levels of expression of the recombinant protein are seen (leaky expression). Which one of the following should be done to minimize such leaky expression?
In a clapp oscillator with three capacitor C1, C2, C3, C1 is used as upper capacitor in voltage divider, C2 is used as lower capacitor in voltage divider and C3 is the third capacitor. For perfect operation which of these capacitor should have minimum value?
In a clapp oscillator with three capacitor C1, C2, C3, C1 is used as upper capacitor in voltage divider, C2 is used as lower capacitor in voltage divider and C3 is the third capacitor. For perfect operation of clapp oscillator value of C3 should be __________
A single 6 V pulse with a width of 600 μs is applied to an integrator consisting of a 150 kΩ resistor and a 0.002 μF capacitor. To what voltage will the capacitor charge?
An integrator consists of a 3.3 kΩ resistor and a 2 μF capacitor. A single 30 V, 6 ms pulse is applied to the input. How much will the capacitor charge?
Why practical integrator is called as lossy integrator?
Figure shows a practical integrator with Rs = 30 M$$\Omega $$, RF = 20 M$$\Omega $$ and CF = 0.1 μF. If a step (DC) voltage of+3 V is applied as input for 0 ≤ t ≤ 4 (t is in second), the output voltage is
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While testing a capacitor with ohm meter, if the capacitor shows charging, but the final resistance reading is appreciably less than normal, it can be concluded that the capacitor is
A parallel plate capacitor has an electrode area of 1000 mm2, with a spacing of 0.1 mm between the electrodes. The dielectric between the plates is air with a permittivity of 8.85∗10-12 F/m. The charge on the capacitor is 100 v. The stored energy in the capacitor is ____________
A 330 pF capacitor and a 220 pF capacitor are each connected across a 6 V dc source. The voltage across the 330 pF capacitor is