Related Questions

The restriction endonuclease HaeIII recognizes the sequence GG↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
The government approved the net neutrality which implies that telecom service providers must treat all internet traffic equally, without any regard to the type, origin or destination of the content. Thus, enabling access to the internet as unrestricted and non-discriminatory. Which of the following is the cause of the statement? I: Restriction of net neutrality allows the internet access service provider to block, degrade, slow down or grant speeds to particular content. II: Restriction of net neutrality affects those who depend on the internet. III: Restriction of net neutrality could result in the discrimination against specific services or websites by the internet access service providers.
The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be
Which of the following are true with regard to anaerobic respiration in bacteria?
P. The final electron acceptor is an inorganic substance other than molecular oxygen.
Q. The number of ATP molecules produced per glucose molecule is more than that produced in aerobic respiration.
R. The number of ATP molecules produced per glucose molecule is less than that produced in aerobic respiration.
S. Only substrate level phosphorylation is used to generate ATP.
Consider the following statements in connection with deflection-type and null-type instruments: 1. Null-type instruments are more accurate than the deflection-type ones. 2. Null-type of instrument can be highly sensitive compared to a deflection-type instrument. 3. Under dynamic conditions, null-type instruments are less preferred to deflection-type instruments. 4. Response is faster in null-type instruments as compared to deflection-type instruments. Which of the above statements are correct?
In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.
A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be
In the conversion of ADP to ATP by the enzyme ATP synthase, which reaction helps in the movement of H+ across the membranes?
Consider the following grammar and the semantic actions to support the inherited type declaration attributes. Let X1, X2, X3, X4, X5 and X6 be the placeholders for the non-terminals D, T, L or L1 in the following table: Production rule Semantic action D → T L X1.type = X2.type T → int T.type = int T → float T.type = float L → L1, id X3.type = X4.type addType(id.entry, X5.type) L → id addType(id.entry, X6.type)   Which one of the following are the appropriate choices for X1, X2, X3 and X4?