Which of the following is a precaution which must be followed while performing assays?

Which of the following is a precaution which must be followed while performing assays? Correct Answer It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added

The precaution to be taken while performing assay is “it should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added.” Following are the beneficial features of biosensor: • The response should be accurate, reproducible, precise, linear and free from electrical noise. • The probe must be tiny and biocompatible. • It should be cheap, small portable and capable of being used by semi-skilled operators.
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Which of the following precautions must not be followed while performing assays?