A recombinant DNA molecule held together by base-pairing although not completely ligated can be introduced into the host cell.

A recombinant DNA molecule held together by base-pairing although not completely ligated can be introduced into the host cell. Correct Answer True

If the complementary homopolymer tails are longer than 20 nucleotides, then quite stable base-paired associations are formed. Once inside the cell the cell’s own DNA polymerase and ligase repair the recombinant DNA molecule.
Bissoy MCQ

Related Questions

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
Consider the following statements regarding cell cycle and choose the correct answer:
1. During G1 phase the cell grows in size and there is an active synthesis of RNA and proteins
2. The third stage of the cell cycle is termed G2 phase
3. In the mitotic phase, the DNA molecule of each chromosome replicates by the synthesis of a new DNA molecule on the template of the existing DNA
Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Vector and insert are mixed, ligated and packaged and introduced into the host by ___________
If a foreign gene is simply ligated into a standard vector, it is likely that a recombinant protein will be produced.
When electroporation is used for introducing DNA into mammalian cell
P. A carrier for DNA is not required.
Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites.
R. The viability of the cell becomes approximately zero percent.
S. The first step involves absorption of DNA on the cell membrane.
In recombinant DNA technology, what molecule is the most commonly used DNA vector (or carrier, used to insert foreign genetic material into an organism’s genome)?
The gaps formed by the hydrolyzing the RNA from RNA:DNA hybrid is filled by DNA polymerase I and the nicks are ligated by T4 ligase.
Consider the following statements regarding the general symptoms of diseases and choose the correct answer:
1. The charges in the host which serve to recognise the disease are called the signs and symptoms of the disease
2. The sign of a disease is the external appearance of some portion of the pathogen of the host
3. The symptoms are the visible effects which the host plant induces on the parasites
4. Any visible deviation on the host plant from the normal structure and function is called a symptom
The integration of viral DNA into the host DNA is promoted by the host integrase enzyme.