Related Questions

Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease
The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.
Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Consider the following statements regarding the DNA-mediated gene transfer and choose the correct answer:
1. DNA fragments carrying the gene to be transferred are mixed with a carrier DNA
2. They are then precipitated out with a calcium phosphate buffer
3. The precipitate is used to transform target cells
4. The Ca-precipitated DNA enters the cell by exocytosis
Which of the following statements are correct? Very long DNA fragments (> 100 kb) from eukaryotic genomes
P. Can be efficiency packaged in a λ phage vector
Q. Can be propagated in yeast artificial chromosomes
R. Must have cohesive ends for cloning
S. Can be separated by pulsed field gel electrophoresis techniques
During storage of DNA fragments for making library, larger fragments are having more chances of getting lost.
Which of the following statements are true with respect to a special complex called 'dicer'?
P. It consists of deoxyribonuclease and DNA fragments.
Q. It consists of ribonuclease and RNA fragments.
R. It is involved in gene silencing.
S. It triggers apoptosis.
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?
A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated?