Stabilization of Enzymes in Biphasic Aqueous-Organic Systems MCQ

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• Which of the following is not an advantage of impregnated wet beads?
  ক

  Protection of the contained enzyme

  খ

  More facile recovery of the biocatalyst

  গ

  Solvent polarity

  ঘ

  Allowing efficient and continuous use of biphasic PBRs
• S-shaped curve may be shifted to the left by immobilizing the enzyme within highly hydrophilic support.
  ক

  True

  খ

  False
• Biphasic systems may be stabilized by the use of __________
  ক

  deuterated water

  খ

  sugar

  গ

  co-factors

  ঘ

  co-enzymes
• Which of the following factor is not important for making the choice of organic solvents in biphasic system?
  ক

  Solvent polarity

  খ

  Ease of recovery

  গ

  Reproducible and linear

  ঘ

  Specific inhibitory effects
• Due to organic phase in biphasic system, the most important factor in the balance between stabilization and inactivation is ______________
  ক

  solvent polarity

  খ

  inactivation of the enzyme

  গ

  stability of the enzyme

  ঘ

  partition coefficient
• In biphasic systems, the limited amount of water available considerably reduces the rate of thermoinactivation.
  ক

  True

  খ

  False
• One major factor that must be addressed for the use of biphasic systems in many enzyme-catalyzed reactions is ____________
  ক

  solvent polarity

  খ

  inactivation of the enzyme

  গ

  stability of the enzyme

  ঘ

  partition coefficient