Modifications in Cloning Methods, Linkers, Adaptors and Cassettes MCQ

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• If linkers are combined with other features such as a selectable marker, it is called as __________
  ক

  cassette

  খ

  modified linker

  গ

  adaptors

  ঘ

  induced linker
• Choose the correct statement for adaptors.
  ক

  They are blunt ended at both the ends

  খ

  They are single stranded at both the ends

  গ

  They may be single stranded at one end and other end may be blunt

  ঘ

  They don’t have extra restriction sites within their sequence
• There is no effect if the insert itself contains the restriction site.
  ক

  True

  খ

  False
• Linkers are often used in cloning. Choose the incorrect statement for linkers.
  ক

  These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence

  খ

  They are blunt ended molecules

  গ

  They are ligated to staggered ended insert molecules by T4 DNA ligase

  ঘ

  After treatment with enzyme, both the ends of the linker are staggered
• Choose the incorrect statement in respect to topoisomerase I.
  ক

  It is used to cleave only one DNA strand

  খ

  It leaves at the recognition sequence –C/TCCTT- and is covalently attached to one end of the cleaved molecule

  গ

  The supercoiling of the DNA strand is altered and then it is sealed again by the enzyme

  ঘ

  It is slower than the normal DNA ligase
• TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?
  ক

  It is based on the fact that a T residue is incorporated at the end of the PCR product

  খ

  ‘A’ residue is incorporated into the end of the vector

  গ

  It gives a low yield

  ঘ

  It is preferred over blunt end ligation
• What is the disadvantage of amplification of using PCR over natural cloning?
  ক

  In PCR, there is no proof reading activity

  খ

  In PCR, small fragments can’t be amplified

  গ

  There is an A incorporated in PCR products, which makes cloning difficult

  ঘ

  PCR takes more time as compared to natural cloning
• What happens if insert DNA is cut with two different restriction enzymes at the ends?
  ক

  It is difficult to insert the fragment

  খ

  The insert can be ligated in any orientation

  গ

  The insert can be ligated in one orientation only

  ঘ

  The amount of product increases
• When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________
  ক

  forced cloning

  খ

  orientation cloning

  গ

  correct cloning

  ঘ

  restricted cloning
• ccdB gene is used at times. Choose the correct statement with respect to this gene.
  ক

  It is a control of cell birth gene

  খ

  It is activated in complex vectors to increase the fraction of recombinants

  গ

  If intramolecular ligation takes place this protein is released

  ঘ

  It is often regarded as control of cell death and birth gene
• What is the correct time for carrying out the alkaline phosphatase treatment?
  ক

  After the cutting of vector has been done

  খ

  Before the insert DNA and the vector are mixed

  গ

  After the cutting of vector has been done but before the insert DNA and vector are mixed

  ঘ

  After the mixing of insert DNA and vector
• Alkaline Phosphatase is used at times and the vector is treated with it. Choose the incorrect statement.
  ক

  It removes 5’ terminal phosphate group from nucleic acids

  খ

  The 5’ phosphate group is required for the ligation to take place

  গ

  Two phosphate bonds should be formed for the complete ligation to take place

  ঘ

  The ligation between vector and insert won’t take place
• At times it is observed that non-recombinant plasmids are more than that of recombinant plasmids. Choose the correct statement in regard to it.
  ক

  It makes easier to get the recombinant plasmids from the mixture

  খ

  The ratio of insert DNA to vector DNA is reduced to get recombinant plasmids

  গ

  Alkaline phosphatase is used to get recombinant plasmids

  ঘ

  Alkaline phosphatase method is less reliable in comparison to the ratio method
• There are various methods to distinguish whether a colony contains a recombinant or not. One such method is __________
  ক

  blue white screening

  খ

  checking whether replication is taking place or not

  গ

  checking the number of copies

  ঘ

  looking for the multiple cloning site