Extraction of Enzymes by Chemical Methods MCQ

Test your knowledge with important Extraction of Enzymes by Chemical Methods MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Extraction of Enzymes by Chemical Methods MCQs at Bissoy

• Which chemical is used for extracting enzymes by osmotic shock treatment?
  ক

  EDTA

  খ

  NaoH

  গ

  SDS

  ঘ

  1.4% buffered sucrose
• The phenomenon wherein the osmotic pressure created due to presence of solutes inside and outside the cells will be equal is referred to as ________
  ক

  isotonic condition

  খ

  hypertonic condition

  গ

  hypotonic condition

  ঘ

  osmotic shock
• Pick odd one out.
  ক

  Triton-X-100

  খ

  Cetyl triethyl ammonium bromide

  গ

  Sodium lauryl sulphate (SLS)

  ঘ

  Cetyl trimethyl ammonium bromide
• Which one of the following is not an example for ionic-detergent?
  ক

  Sodium dodecyl sulphate (SDS)

  খ

  Tween

  গ

  Sodium lauryl sulphate (SLS)

  ঘ

  Cetyl trimethyl ammonium bromide
• ___________ is a divalent chelator which can be used to extract enzymes from different sources.
  ক

  Sucrose

  খ

  NaOH

  গ

  Lysozyme

  ঘ

  EDTA
• The enzyme which specifically catalyzes β (1 → 4) glycosidic linkages of NAM (N-acetyl muramic acid) and NAG (N-acetyl glucosamine) present in bacterial peptidoglycan is _______________
  ক

  protease

  খ

  lipase
• Alkali treatment is not advisable for which of the following?
  ক

  Extracellular enzymes

  খ

  Bacterial enzymes in cell wall

  গ

  Enzymes in cell wall of plant sources

  ঘ

  Membrane bound enzymes in eukaryotic cell
• Extraction of enzymes by physical methods include the usage of alkalis, EDTA, lysozyme, etc.
  ক

  True

  খ

  False
• The techniques which adopts the hypertonic and hypotonic condition for cell lysis is referred to as ____________
  ক

  EDTA treatment

  খ

  Ultrasonification

  গ

  Osmotic shock

  ঘ

  Homogenization
• __________________ is an example of anionic detergent.
  ক

  Tween

  খ

  Cetyl triethyl ammonium bromide

  গ

  Sodium dodecyl sulphate (SDS)

  ঘ

  Triton-X-100
• Which of the following is cationic detergent?
  ক

  Sodium dodecyl sulphate (SDS)

  খ

  Cetyl triethyl ammonium bromide

  গ

  Sodium lauryl sulphate (SLS)

  ঘ

  Triton-X-100
• In ___________ treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.
  ক

  Lysozyme

  খ

  EDTA

  গ

  Detergent

  ঘ

  NaoH
• Gram positive bacteria are highly susceptible to lysozyme treatment.
  ক

  True

  খ

  False
• ____________ is an enzyme, which is highly produced by egg white and lachrymal glands.
  ক

  Amylases

  খ

  Lysozyme

  গ

  Invertase

  ঘ

  Protease
• In ________ treatment, produced basic radicals mediate a nucleophilic attack on compounds of cell wall on membrane leading to destabilization and fragmentation of membrane followed by release of enzymes.
  ক

  EDTA

  খ

  Osmotic shock

  গ

  Alkali

  ঘ

  Detergent
• Which of the following is not a technique for the extraction of enzymes by chemical methods?
  ক

  Alkali treatment

  খ

  Osmotic shock

  গ

  Detergent treatment

  ঘ

  Homogenization