DNA Cloning: The Basics-1 MCQ

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• Repressor molecules bind to __________
  ক

  Operator

  খ

  Promoter

  গ

  Enhancer

  ঘ

  Hormone response element
• Which of the following enzyme is required for end to end joining of DNA?
  ক

  DNA ligase

  খ

  Restriction endonuclease

  গ

  RNA polymerase

  ঘ

  DNA polymerase
• What is a DNA library?
  ক

  A DNA fragment inserted into a vector

  খ

  A general collection of all genes sequenced thus far

  গ

  All DNA fragments identified with a probe

  ঘ

  A collection of DNA fragments that make up the entire genome of a particular organism
• In which stage of genetic engineering a probe is used?
  ক

  Cleaving DNA

  খ

  Recombining DNA

  গ

  Cloning

  ঘ

  Screening
• E.cor1 is a __________
  ক

  DNA ligase enzyme

  খ

  Restriction endonuclease

  গ

  A vector used for insulin synthesis

  ঘ

  A plasmid used as a vector
• The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________
  ক

  Chromosome

  খ

  Plasmid

  গ

  Genomic DNA

  ঘ

  Bacteriophage
• To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?
  ক

  MgCl2

  খ

  CaCl2

  গ

  NaCl

  ঘ

  HCl
• Generally a plasmid vector contains how many elements?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?
  ক

  Alkaline phosphatase and terminal transferase

  খ

  Exonuclease III and alkaline phosphatase

  গ

  Bacteriophage lambda exonuclease and terminal transferase

  ঘ

  Exonuclease III and terminal transferase
• Which of the following is true about restriction endonucleases?
  ক

  Type I and II requires ATP to move along DNA

  খ

  Type I, II and III requires ATP to move along DNA

  গ

  Type II requires no ATP and cleaves DNA within recognition sequence

  ঘ

  Type II requires ATP and cleaves DNA within recognition sequence
• Which of the following can be termed as a restriction modification system?
  ক

  Restriction endonuclease + methylase

  খ

  DNA ligase + methylase

  গ

  Restriction endonuclease + acetylase

  ঘ

  DNA ligase + acetylase
• Which of the following enzyme is responsible for making a DNA copy from RNA?
  ক

  Reverse transcriptase

  খ

  DNA polymerase

  গ

  RNA polI

  ঘ

  RNA polII
• Which of the following does not act as a restriction enzyme?
  ক

  EcorI

  খ

  BamHI

  গ

  HindIII

  ঘ

  polydeoxyribonucleotide synthase