Cultured Cells in Mammals and Restricted Areas in Intact Organisms MCQ

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• The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.
  ক

  depends only on, not depends

  খ

  depends, depends also

  গ

  doesn’t depends, but depends

  ঘ

  neither depends, nor depends
• There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.
  ক

  cysteine

  খ

  methionine

  গ

  either cysteine or methionine

  ঘ

  cysteine and methionine both
• Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).
  ক

  inducible, short terminal repeat region

  খ

  inducible, long terminal repeat region

  গ

  constitutive, short terminal repeat region

  ঘ

  constitutive, long terminal repeat region
• Expression of tTA gene is ________ by doxycycline which is _______ analogue.
  ক

  activated, tetracycline

  খ

  inactivated, chloramphenicol

  গ

  activated, chloramphenicol

  ঘ

  inactivated, tetracycline
• Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).
  ক

  True

  খ

  False
• In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.
  ক

  presence, activates

  খ

  absence, activates

  গ

  absence, inactivates

  ঘ

  presence, inactivates
• Constitutive promoters are majorly obtained from ________
  ক

  fungi

  খ

  bacteria

  গ

  mammalian cells

  ঘ

  viral cells
• Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.
  ক

  alpha satellite

  খ

  beta satellite

  গ

  satellite 2

  ঘ

  satellite 1
• Choose the incorrect statement in respect to MACs.
  ক

  They are mammalian or human artificial chromosomes

  খ

  Large stretches of DNA (in excess of 100 k[ can be inserted

  গ

  A selectable marker is required

  ঘ

  It can be maintained for long term
• The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?
  ক

  Deletion of the packaging signal in the helper genome

  খ

  Deletion of multiple parts of the helper genome

  গ

  Division of the helper genome into several pieces

  ঘ

  All of the mentioned
• LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.
  ক

  eukaryotic plasmid

  খ

  prokaryotic plasmid

  গ

  either eukaryotic or prokaryotic plasmid

  ঘ

  yeast plasmid
• It is beneficial to use retrovirus for genetic manipulation of cells in the culture.
  ক

  True

  খ

  False
• Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.
  ক

  DNA polymerase, nucleus

  খ

  DNA polymerase, cytoplasm

  গ

  RNA polymerase, cytoplasm

  ঘ

  RNA polymerase, nucleus
• Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.
  ক

  200kb, 25kb

  খ

  1000kb, 200kb

  গ

  500kb, 50kb

  ঘ

  800kb, 100kb
• T protein is required for replication. It is a product of ____________
  ক

  delayed early transcription

  খ

  late transcription

  গ

  early transcription

  ঘ

  both early and late replication
• Splicing of the transcripts is necessary for efficient expression.
  ক

  True

  খ

  False
• SV40 is a virus and it produces how many transcripts?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• In ___________ cells, virus replication doesn’t take place and viral DNA ___________
  ক

  non-permissive, is also not expressed

  খ

  permissive, is also not expressed

  গ

  non-permissive, can be expressed

  ঘ

  non-permissive, is always expressed
• Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________
  ক

  protoplast

  খ

  ghosts

  গ

  shrunken cells

  ঘ

  vacuole
• DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______
  ক

  polysaccharide, negatively

  খ

  polysaccharide, positively

  গ

  monosaccharide, positively

  ঘ

  monosaccharide, negatively
• A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________
  ক

  kidney

  খ

  liver

  গ

  lymphoblast from leukaemia patient

  ঘ

  both kidney and lymphoblast from a leukaemia patient
• Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme.
  ক

  more efficiently

  খ

  less efficiently

  গ

  with same efficiency

  ঘ

  either with same or more efficiency
• Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol.
  ক

  True

  খ

  False
• Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.
  ক

  two, nucleoside

  খ

  two, nucleotide

  গ

  one, nucleotide

  ঘ

  one, nucleoside
• Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________
  ক

  DNA damaging agent

  খ

  DNA synthesis promoter

  গ

  Inhibiting RNA synthesis

  ঘ

  Activating RNA synthesis
• Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene.
  ক

  streptococcal

  খ

  bacilovirus

  গ

  streptomyces

  ঘ

  both streptococcal and streptomyces
• Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________
  ক

  initiating protein synthesis

  খ

  inhibiting protein synthesis

  গ

  initiating DNA binding process

  ঘ

  inhibiting DNA binding process
• Cells deficient in thymidine kinase (TK) are also killed in HAT medium.
  ক

  True

  খ

  False
• Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.
  ক

  lithium acetate

  খ

  calcium phosphate

  গ

  sodium chloride

  ঘ

  aluminum sulphate
• Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?
  ক

  Hypoxanthine and thymidine

  খ

  Thymidine

  গ

  Aminopterin and thymidine

  ঘ

  Hypoxanthine, thymidine and aminopterin (HAT medium)