Cloning in Lambda MCQ

Test your knowledge with important Cloning in Lambda MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Cloning in Lambda MCQs at Bissoy

• There are some phages which don’t preferentially transduce some special regions of the phage genome. These phages are known as __________
  ক

  transducing phages

  খ

  specialized transducing phages

  গ

  generalized transducing phages

  ঘ

  transforming phages
• There is a limit on upper size of the DNA to be packed. Choose the correct statement with respect to phages in this context.
  ক

  There is some phage DNA lost in this process

  খ

  The phages are known as transformed phages

  গ

  These type of phages can’t be selected and harvested

  ঘ

  Lambda is not a special attachment site
• The replication rate remains the same for all the phages irrespective of what sequence is there in the phage.
  ক

  True

  খ

  False
• The essential sites for recombination are known as __________
  ক

  chi sites

  খ

  rec sites

  গ

  gam sites

  ঘ

  red sites
• Choose the correct statement for RecBCD nuclease.
  ক

  It promotes rolling circle replication

  খ

  It is blocked by Gam protein

  গ

  It blocks theta mode of replication

  ঘ

  It is blocked by Red protein
• Phages which are designated as spi-are __________
  ক

  red+ gam+

  খ

  red+ gam-

  গ

  red- gam+

  ঘ

  red- gam-
• The red and gam genes are removed in which type of phages?
  ক

  Substitution phage

  খ

  Replacement and substitution phages both

  গ

  Replacement phage

  ঘ

  Substitution is preferred over replacement phage
• What is the function of red and gam gene products?
  ক

  It promotes the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P1

  খ

  It inhibits the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P2

  গ

  It inhibits the growth of the phage in the E. coli cells which are lytic for bacteriophage P1

  ঘ

  It activates the growth of the phage in the E. coli cells which are lytic for bacteriophage P2
• The cI function can be scanned in order to check whether the recombinants are present or not. Which of the following doesn’t hold true?
  ক

  The cI protein is required for the formation of lysogen

  খ

  The plaques formed are turbid in the case if the cI gene is active

  গ

  The plaques formed are clear in the case if the cI gene is inactive

  ঘ

  hflA mutant host reduces the amount of cII stability
• If a single system is used for packaging, there are increased chances of endogenous material being packed.
  ক

  True

  খ

  False
• Which of the following is not a consequence of deletion in the b region?
  ক

  Excision of the prophage on induction is prevented

  খ

  It reduces the amount of endogenous phage DNA in the packaging mix

  গ

  It reduces the amount of exogenous phage DNA in the packaging mix

  ঘ

  It reduces the level of background non-recombinant phage
• Which of the following is used for blocking the phage-encoded recombination?
  ক

  Mutation in D gene

  খ

  Mutation in E gene

  গ

  Red mutation

  ঘ

  Mutation in S gene
• What happens if chain-termination mutation is in the S gene?
  ক

  Cell lysis is blocked

  খ

  Growth of cells containing low levels of packaging proteins is not allowed

  গ

  The lysis of cells is not carried artificially

  ঘ

  Packaging is not carried out efficiently
• Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________
  ক

  A gene

  খ

  D gene

  গ

  E and D gene

  ঘ

  D and A gene
• Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.
  ক

  Separate strains with chain-termination mutations in different genes for coat components are used __________

  খ

  Temperature sensitive cI repressor is used

  গ

  Due to heat shock packaging proteins are induced

  ঘ

  Packaging is carried out in each strain
• Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.
  ক

  Packaging in phage coat is a more efficient process than the normal transformation process

  খ

  The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration

  গ

  Lysate constitutes of lambda proteins needed for phage assembly

  ঘ

  The amount of non-recombinant phage in the background is less
• Physical separation is also used at times. Choose the correct statement with respect to this method.
  ক

  Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

  খ

  In sugar density gradient, the molecules are separated on the basis of density

  গ

  The central portion is recovered from the gel or the gradient

  ঘ

  Gel is more efficient than the sugar density gradient
• At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.
  ক

  It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there

  খ

  In this method, the central portion is cut with another enzyme after being separated from the arms

  গ

  The arms are also cut with this second enzyme

  ঘ

  It is not necessary to remove the fragments generated
• Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated.
  ক

  True

  খ

  False
• Choose incorrect statement for the substitution vector EMBL4.
  ক

  It is having two single multiple cloning sites

  খ

  The region to be removed is on one side of the multiple cloning sites

  গ

  If a digestion within the multiple cloning sites, it generates central portion and right and left arms

  ঘ

  The right and left portions are called as arms
• The fragment inserted in the place of the central portion of the genome is known as __________
  ক

  insertion fragment

  খ

  substitution fragment

  গ

  stuffer fragment

  ঘ

  displacement fragment
• Choose the incorrect statement for the central portion of the lambda genome.
  ক

  The central portion is not required for lytic growth

  খ

  The central portion includes repressor gene

  গ

  The repressor gene is not required for lysogenic growth

  ঘ

  It is approximately 10kbp long
• A portion of phage is removed and in place of it, the DNA of interest is inserted. This type of vector is called as __________
  ক

  displacement vector

  খ

  insertion vector

  গ

  substitution vector

  ঘ

  transposition vector
• The presence of insert leads to inactivation of which gene?
  ক

  cII

  খ

  cI

  গ

  cIII

  ঘ

  both cII and cIII
• Choose the correct statement for lambda gt10.
  ক

  It is having an EcoRI site

  খ

  It is having cII gene

  গ

  It accepts an insert of size greater than that of 8 kbp

  ঘ

  This size is less than that the size which is generally accepted by wild type phage
• Insertion vectors are a type of vectors. Choose the correct statement for this type of vector.
  ক

  These are complex vectors

  খ

  DNA is inserted through many restriction sites

  গ

  There is an upper limit on the size of the DNA to be packaged

  ঘ

  Lambda gt09 is an example of this type of vector